Next Generation Sequencing Facilitates Quantitative Analysis of HHSteCs and HHSteC treated rhCYGB [HSC]

Purpose: Hepatic stellate cells (HSCs) plays an important role in liver fibrosis. Recombinant human Cytoglobin (rhCYGB) can inhibit the activation of HSCs. The goal of this study is to investigate the rhCYGB targeted signalling pathways regulating the activation of HSCs. Method: mRNA profiles of HHSteCs control and HHSteCs treated with rhCYGB (80 µg/ml, for 48 hour) were generated by deep sequencing. Results: The RNA-seq data confirmed that extracellular matrix-encoding genes and fibrosis-related genes were down-regulated by the His-CYGB treatment. The top of up-regulated genes following His-CYGB treatment were interferon (IFN)-stimulated genes (ISGs), implying IFN signaling in His-CYGB treatment. mRNA profiles of HHSteCs with or without rhCYGB treatment