The effects of shaking duration on the abundance and the community of aerobic denitrifying bacteria in shrimp pond water and sediment samples

With the rapid development of intensive aquaculture, the considerable release of nitrogenous organic compounds has become a serious threat to aquatic organisms. Currently, isolating autochthonous aerobic denitrifying bacteria (ADB) from aquaculture environments is essential for the biological elimination of nitrogenous pollutants. In this study, the enrichment of ADB from shrimp pond water and sediment samples was conducted under different shaking durations to explore the enrichment efficiency. The absolute abundance of total bacteria, nosZ-type, and napA-type ADB was measured using RT-qPCR. High-throughput sequencing of 16s RNA, napA and nosZ was performed to reveal the community structure of bacteria and ADB, respectively.