Efficiency of URA3 marker deletion by HR.
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For URA3 selection marker reuse, the strains integrated with deletion or integration cassette containing URA3 marker were grown overnight in the YPD liquid medium and then plated on the 5-FOA selection medium. The percentage of URA3 marker deletion is shown and the number of total colonies screened is included in parentheses. The experiments were performed in duplicate.
创建时间：
2018-03-23
