Gene expression of mouse embryonic gonad [Nanos2_Stra8]

To examine whether the meiosis was solo factor responsible for the disruption of the male gonocyte differenitiation in the Nanos2-/- male germ cells, we deleted Stra8 function from Nanos2-null background and performed expression microarray analysis of the Nanos2+/-/Stra8+/- , Nanos2-/-/Stra8+/-, and Nanos2-/-/Stra8-/- male embryonic gonads at E14.5. Biological duplicates were examined at each stage, genotype, and sex for each experiment.为探究减数分裂是否是引发Nanos2纯合敲除（Nanos2-/-）雄性生殖细胞中雄性性原细胞分化异常的唯一因素，我们在Nanos2基因缺失背景中敲除了Stra8基因的功能，并对胚胎发育第14.5天（E14.5）的三类基因型雄性胚胎性腺——Nanos2+/-/Stra8+/-、Nanos2-/-/Stra8+/-及Nanos2-/-/Stra8-/-——进行了基因表达芯片（expression microarray）分析。本实验针对各发育阶段、基因型与性别均设置了生物学重复。