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FAD2 PERFORMANCE LOCI AND CORRESPONDING TARGET SITE SPECIFIC BINDING PROTEINS CAPABLE OF INDUCING TARGETED BREAKS

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国家林业和草原科学数据中心2023-02-12 更新2024-03-07 收录
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PROBLEM TO BE SOLVED: To provide compositions and methods for modifying and/or regulating the expression of the FAD2 gene in plants, comprising the production of plants in which a desired transgene is targeted and inserted, in the FAD2 (fatty acid desaturase 2) locus involving in fatty acid biosynthesis. SOLUTION: Provided is a method for modifying the genome of a plant cell, the method comprising: cleaving, in a site specific manner, through the use of nucleases binding to any target site of a particular nucleotide sequence in the FAD2 gene in a plant cell, thereby generating a cleavage site in the FAD2 gene, and the FAD2 gene being modified by incorporating an exogenous nucleic acid sequence into the cleavage site after the cleavage. SELECTED DRAWING: None
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国家林业和草原科学数据中心
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2023-02-12
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