The 153 readable DNA sequences of a diet research on Eurasian otter of Kinmen Island
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https://datadryad.org/dataset/doi:10.5061/dryad.msbcc2fz2
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资源简介:
It is well known that Eurasian otters principally feed on fishes and
crustaceans, however, their detailed diet taxonomies are not fully
understood. I performed a series of Sanger-sequencing reactions, utilizing
nine primer sets for Eurasian otter diet research. In these data
materials, in total 153 readable DNA sequences of a diet research on
Eurasian otter of Kinmen Island are presented. Such information provides a
very detailed insight of feeding behavior of the Eurasian otter in Kinmen.
These sequences were sequenced from 49 DNA samples extracted from fresh
spraints collected from Kinmen from April 2017 to November 2018. Each DNA
sample was PCR-amplified 9 times and with 9 primer sets. Eight of them are
published universal COI primers and targeted toward different prey taxa.
Besides, I designed a group of COIII primers for the introduced tilapia,
the most abundant prey species in Kinmen Island. In total, 16 prey types
were identified across all 49 samples. Furthermore, another identified
sequence group comprised “non-food species”, including bacteria, amoeba,
diatoms, small insects (e.g. ants and mosquitos) and small
invertebrates, as well as the otters’ sequences themselves. The detailed
information of these 153 sequences including collection sites, primers,
identified food/non-food species, linkage of blast results, etc. are
listed in S2.xlxs; for the sequences themselves are listed in S3.txt.
提供机构:
Dryad
创建时间:
2021-05-24



