Apparent Dissociation Constants for the Ub Binding Affinities of Tandem AT3-UIM12, Single AT3-UIM Motifs and Their Mutants.

aThe dissociation constants were determined from NMR titration of different GB1-tagged UIM peptides to 15N-labeled Ub. For comparison, we calculated the apparent KD,app values for AT3-UIM12, provided that the two UIM motifs bind Ub equally.
bThe chemical shift changes of three individual residues I13, K48 and L71 on Ub were used to calculate the dissociation constants. Data are presented as mean ± SD.
cThe sequences of the linker regions for wild-type AT3-UIM12 and its mutants. WT: 239EIDMED244; GS-Sub: GSSGGS; GS-Ins: EIDGSSGGSMED; RAP80-Linker: EAREVNNQEEK.