Outputs from pairwise T-tests conducted between stage 15 (n = 7) and stage 15 ZZf samples suspected not to have undergone sex reversal (n = 2).

The normalised transcripts per million (TPM) for six genes, three male (AMH, DMRT1, SOX9) and three female genes (FOXL2, CYP19A1, CYP17A1) were used. The two samples suspected of not undergoing sex reversal show significantly different expression levels for four of these genes, and differences just above the significance threshold of ≤0.05 for the other two genes. On this basis, these two samples were removed from further analysis. (XLSX)