Gene deletion and reaction addition strategies identified by SimOptStrain for glycerol production under glucose aerobic condition.

aEnzyme Commission number.3.1.3.21 sn-Glycerol 3-phosphate+H2OGlycerol+Orthophosphate.2.7.1.142 sn-Glycerol 3-phosphate+D-GlucoseGlycerol+D-Glucose 6-phosphate.2.1.3.1 Malonyl-CoA+PyruvateAcetyl-CoA+Oxaloacetate.bTheoretical maximum production is reported as mol glycerol produced/mol glucose consumed for each strain with non-native reaction additions, but without gene deletions. A maximum glucose uptake rate of 10 mmol gDW−1 h−1 and a maximum oxygen uptake rate of 18.5 mmol gDW−1 h−1 were used.cYield is reported as % of the TMP for each strain after reactions are added.dBest strategies without addition of non-native reactions (no strategy was found for k = 5 and k′ = 0 because any small production increases (over the k = 3 strategy) were negated by the penalty α = 10−6).