Transcription profiling of wild type and Rpe65-/- mouse retinae to characterize gene response in Rpe65-/- mouse model of Lebers congenital amaurosis during progression of the disease

To characterize gene response in RPE65-/- mouse model of Leber's congenital amaurosis during progression of the disease, we analyzed differential gene expression in retinae early in the development of the disease, namely before and at the onset of photoreceptor cell death in knock-out mice of 2, 4 and 6 months of age. Experiment Overall Design: We compared gene expression in wild type and RPE65-/- retinae at 2, 4 and 6 months of age. Three biological replicates were performed for each of the six conditions analyzed; this series hence contains 18 samples.为了表征Leber先天性黑蒙（Leber's congenital amaurosis）RPE65基因敲除（RPE65-/-）小鼠模型在疾病进程中的基因应答特征，我们针对疾病早期发育阶段——即2、4、6月龄基因敲除小鼠感光细胞死亡发生前及发生时的视网膜组织，开展了差异基因表达（differential gene expression）分析。实验整体设计：本研究比较了2、4、6月龄野生型与RPE65-/-小鼠视网膜的基因表达水平。本次分析涵盖6种实验条件，每种条件均设置3次生物学重复，因此该数据集共包含18个样本。