Fluorescent Macrophages in Drosophila Embryo

This is a data set that contains a time sequence of fluorescently labelled macrophages that migrated in a drosophila embryo.

The macrophages were visualised in the  embryo by using the UAS/GAL4 system.We used the srpHemo-Gal4driver,which mediate the expression of genes downstream of a UASsequence specifically in macrophages to express the following UAS fluorescent probes: UAS-RedStinger for the nuclei and UAS-Clip-GFP for the microtubules.

Details of the imaging and preparation have been published in:

Evans, I.R.; Zanet, J.; Wood, W.; Stramer, B.M. Live imaging of Drosophila melanogaster embryonic339hemocyte migrations.Journal of visualized experiments: JoVE2010,36.

Details on the segmentation, tracking and analysis of the macrophage migration have been published in:

José Alonso Solís-Lemus, Besaiz J Sánchez-Sánchez, Stefania Marcotti, Mubarik Burki, Brian Stramer, Constantino Carlos Reyes-Aldasoro, Comparative study of contact repulsion in control andmutant macrophages using a novel interaction detection, Journal of Imaging, BioRxiv,  https://doi.org/10.1101/2020.03.31.018267

and

Solís-Lemus, J.A.; Stramer, B.; Slabaugh, G.; Reyes-Aldasoro, C.C. Macrosight: A Novel Framework to Analyze the Shape and Movement of Interacting Macrophages Using Matlab.Journal of Imaging 2019,5