Impact of 6-mercaptopurine on LPS treated primary cultures of enteric nervous system. Impact of 6-mercaptopurine on LPS treated primary cultures of enteric nervous system

Enteric nervous system is involved in the regulation of intestinal inflammation. We developped mouse primary cultures of enteric nervous system to study impact of LPS, as pro-inflammatory mediator, and of the pro-drug 6-mercaptopurine on enteric inflammatory pathways We used microarrays to detail the global programme of gene expression underlying enteric neuro-inflammation and identified classes of up-regulated genes during this process. Overall design: Mouse ENS primary cultures were treated with LPS in presence or absence of 6-mercaptopurine. Genome-wide expression analysis was performed using Clariom S microarray