Mus musculus Targeted Locus (Loci)
收藏NIAID Data Ecosystem2026-03-11 收录
下载链接：
https://www.ncbi.nlm.nih.gov/sra/SRP049176
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资源简介：
To study the effects of post-testicular oxidative damage on mammalian sperm structure and function we generated the gpx5-/- mouse strain a transgenic mouse model lacking glutathione peroxidase-5, a key epididymal luminal antioxidant scavenger. In the present study we have attempted to further characterize the mouse sperm chromosomal regions and DNA sequences most sensitive to post-testicular oxidative damage. To do so, we carried out a sperm DNA immunoprecipitation scheme (hereby designed as the OxiDIP protocol) using an anti-8-OHdG antibody that specifically recognizes oxidized guanine residues, a classical signature of DNA oxidative damage in wild-type and gpx5-/- mouse sperm. OxiDIP recovered DNAs were subjected to next generation sequencing on the Illumina Hi-Seq 2500 platform.
创建时间：
2020-05-21
