Gene expression of zebrafish larvae upon treating with lalistat or U18666A. Gene expression of zebrafish larvae upon treating with lalistat or U18666A

To test if HIF pathway is regulated by lipids in zebrafish larvae, we have employed whole genome microarray expression profiling as a discovery platform to identify induced genes upon lalistat or U18666A treatment. Lalistat is a specific inhibitor for lysosomal acid lipase (LAL), which is required for break down triglyceride or cholesterol ester to release free fatty acids. U18666A is a specific inhibitor for NPC, which is required for export free cholesterol oout of lysosomes. Overall design: Zebrafish larvae (5dpf) were treated with vehicle control DMSO, lalistat (25uM) or U18666A(2uM) for 24hr. Each RNA sample were extracted from pooled 4 larvae. Three biological replicates (3 clutches from different pairs of WT zebrafish mating) were conducted.