Interleukin-10/CSF1R fusion protein rejuvenates the exhausted T cells for executing antitumor activity III. Interleukin-10/CSF1R fusion protein rejuvenates the exhausted T cells for executing antitumor activity III

Bulk RNA sequence (RNA-seq) has been used to explore the effect of IL10/CSF1R fusion protein modulation in tumor microenvironment. Four groups were analyzed which contain Ctrl, CSF1R, IL10 and BF10. MTC-Q1 oral cancer cells were orthotopic injection in C57BL/6 mice. CD45-negatve cells has been isolated and RNA were extracted by Trizol, followed by cDNA were sequenced by Illumina NextSeq 550. Overall design: Examination of therapeutic modulation of IL10/CSF1R fusion protein within tumor microenvironment in mice cancer model. RNA-seq of CD45-negatve cells from orthotopic cancer tissue after four dose treatments.