RNA sequencing on liver samples from wild type C57BL/6J mice exposed to the vandetanib

Purpose: The aim of this study was to demonstrate the hepatotoxicity mechanism induced by vandetanib. Methods: Liver mRNA profiles of C57BL/6J mice exposed to the vandetanib were generated by RNA sequencing. Results: We analyzed the results of high-throughput transcriptome sequencing on liver samples from C57BL/6J mice exposed to the vandetanib and identified that vandetanib increased apoptosis-related genes expression apoptosis and the JAK-STAT signaling pathway was significantly enriched. Overall design: RNA was isolated from mice administered with CMC-Na or 100 mg/kg/day vandetanib using the TRIzol reagent (15596026, Invitrogen). The subsequent RNA sequencing (RNA-seq) was performed by LC-Bio.