Data for isolation-by-environment and its consequences for range shifts with global change: Landscape genomics of the invasive common tansy

Invasive species are a growing global economic and ecological problem. However, it is not well understood how environmental factors mediate invasive range expansion. In this study, we investigated the recent and rapid range expansion of common tansy across environmental gradients in Minnesota, U.S.A. We densely sampled individuals across the expanding range and performed reduced representation sequencing to generate a dataset of 3071 polymorphic loci for 176 individuals. The dataset includes additional samples from the native range in Finland that were not used in the downstream analysis but are contributed for completeness. The dataset includes the genotype calls for all individuals sampled and sequenced. The genotype file was generated by stacks2.59 running the denovo pipeline and then using the populations function where we kept loci that were in 70% of populations and had a minor allele frequency of at least 1%. We used non-spatial and spatially-explicit analyses to determine the re..., 1. Description of methods used for collection/generation of data:
 
Samples were processed from leaf tissue that was collected fresh at the sampling locality and then immediately placed in a bag of silica gel and labeled. Locality information for each sample was taken using a handheld GPS unit. Genotypes were generated from GBS Illumina sequencing at UMN Genomic Center (UMGC).  Samples were processed at the University of Minnesota Genomics Center (https://genomics.umn.edu/services/gbs) using Illumina NextSeq sequencing using the following protocol. UMGC created dual-indexed GBS libraries using the enzyme combination BamHI + NsiI. Enzyme selection followed from a small pilot study used to assess the proper enzyme combination to produce approximately 5000-10000 loci for the average read depth of approximately 1 million per individual. Briefly, extracted DNA was quantified using Picogreen ® (Thermofisher Scientific, MA, USA) and normalized to 10 ng/µl. A total of 100 ng of DNA per sample w..., , # Data for Isolation-by-Environment and its consequences for range shifts with global change: landscape genomics of the invasive common tansy

[https://doi.org/10.5061/dryad.h70rxwdsk](https://doi.org/10.5061/dryad.h70rxwdsk)

##### Author Information

Author Contact: Ryan Briscoe Runquist ([[e-mail]](mailto:[e-mail]))
Principal Investigator Contact Information

Name: Ryan Briscoe Runquist
Institution: University of Minnesota
Address: Department of Plant and Microbial Biology, 1479 Gortner Ave, 140 Gortner Laboratory, St. Paul, MN 55108
Email: [[e-mail]](mailto:[e-mail])
ORCID: [https://orcid.org/0000-0001-7160-9110](https://orcid.org/0000-0001-7160-9110)

Associate or Co-investigator Contact Information
Name: David A. Moeller
Institution: University of Minnesota
Address: Department of Plant and Microbial Biology, 1479 Gortner Ave, 140 Gortner Laboratory, St. Paul, MN 55108
Email: [[e-mail]](mailto:[e-mail])
ORCID: [https://orcid.org/0000-0...