HDX-MS analysis of the DNA-mKu interaction and dynamics

This project aims to decipher the DNA-protein interactions of the DNA end-binding protein mKu. In Mycobacterium tuberculosis and prokaryotes in general, Ku serves as the initiator of the non-homologous end-joining (NHEJ) DNA repair pathway. Using HDX-MS complemented with structural data, we investigate critical contacts at the DNA-protein interface and explore the dynamic behavior of the complex in solution.