The LC3 conjugation machinery specifies the loading of RNA-binding proteins into extracellular vesicles (lrgRNA-seq dataset)

We employed RNA sequecing to profile large RNAs (mRNAs and lincRNAs) packaged into extracellular vesicles (EVs) from WT cells and cells deficient for the LC3 conjugation machinery EVs from wild-type, ATG7-/- and ATG12-/- HEK293T cells and corresponding cell samples were collected after 24h of serum starvation. Total RNA from wild-type, ATG7-/- and ATG12-/- HEK293T EVs and cells was isolated and used for the preparation of uniquely barcoded large RNA sequencing libraries (n=3). Results of single-end 50 base pair sequencing on two separate lanes of a HiSeq4000 for these samples revealed negligible differences in the large RNA profiles of EVs from ATG7-/- and ATG12-/- cells relative to EVs from wild-type cells. These results suggest that large RNAs are packaged into EVs via mechanisms independent of the LC3 conjugation machinery.