Traffic-related diesel pollution particles impair the lysosomal functions of human iPSC-derived microglia

This repository contains the data and the code used in Ohtone Sohvi's TUBE project.

A folder can contain the starting raw data in "data", the R scripts in order of execution (op1, op2 ..) and the "output" folder that contains the final processed data of each operation.

"*_analysis" contains the processing and analysis of the sequencing data. It is a module implementation, so op1 script runs, then op2, then op3 and so on.

"*_counts" contains the results of processing the fasta sequences with nfcore rnaseq workflow. It allows to get the raw values and to replicate the fasta processing.

Original fasta files of this project are 500GB and are available upon request due to limited cloud space.