HER2 aptamer-conjugated iron oxide nanoparticles with PDMAEMA-b-PMPC coating for breast cancer cell identification - supplementary data

<b>Aim: </b>To synthesize HER2 aptamer-conjugated iron oxide nanoparticles with a coating of poly(2- (dimethylamino) ethyl methacrylate)–poly(2-methacryloyloxyethylphosphorylcholine) block copolymer (IONPPPs). <b>Methods: </b>Characterization covered molecular structure, chemical composition, thermal stability, magnetic characteristics, aptamer interaction, crystalline nature and microscopic features. Subsequent investigations focused on IONPPPs for <i>in vitro </i>cancer cell identification. <b>Results: </b>Results demonstrated high biocompatibility of the diblock copolymer with no significant toxicity up to 150 μg<b>/</b>ml. The facile coating process yielded the IONPP complex, featuring a 13.27 nm metal core and a 3.10 nm polymer coating. Functionalized with a HER2-targeting DNA aptamer, IONPPP enhanced recognition in HER2-amplified SKBR3 cells viamagnetization separation. <b>Conclusion: </b>These findings underscore IONPPP’s potential in cancer research and clinical applications, showcasing diagnostic efficacy and HER2 protein targeting in a proof-of-concept approach.