Short and long read sequencing of human mammary epithelial MCF10a-Snail-ER cells after Epithelial-to-Mesenchymal Transition initiation

We cultured MCF10a-Snail-ER cells and induced EMT initiation with tamoxifen. A matched sequencing of their PolyA RNA was performed, using Illumina and direct RNA Oxford Nanopore sequencing technologies. Both generated datasets supported the development of hybrid bioinformatics tools. Overall design: Parallel sequencing of matched MCF10a-Snail-ER PolyA RNA samples using Illumina and direct RNA Oxford Nanopore technologies.