Mitochondrial Genome Assembly and Annotation of Ophiostoma novo-ulmi Subspecies

This dataset includes the assembled and annotated mitochondrial genomes of Ophiostoma novo-ulmi subsp. americana (strain S182) and subsp. novo-ulmi (strain H328). Genomic DNA was extracted from fungal mycelium, and whole-genome sequencing was performed on an Illumina platform (Novogene, Germany), generating 150 bp paired-end reads. Mitochondrial genomes were assembled using GetOrganelle and NovoPlasty, resulting in genome sizes of 56,827 bp for americana and 59,857 bp for novo-ulmi. Assembly showed high organelle coverage: for H328, 556,570 reads were assembled from 12,791,200 total reads (4.58% organelle genome, average coverage 1,478×), while for S182, 570,502 reads were assembled from 15,001,188 total reads (3.98% organelle genome, average coverage 1,590×). Genome annotation was performed using MFAnnot, MITOS, and GeSeq. Comparative analyses with mitochondrial genomes from additional Ophiostoma species revealed high variability, particularly in intron content.