Comparison of wild type and abc1k6-1 mutant total leaf extracts from 4 and 5 week old plants

The quantitative proteomes of leaf tissue from A. thaliana at 4 weeks and 5 weeks of growth using tandem mass spectrometry with label-free quantification. A comparison of three genotypes has been performed, namely i) the Col-0 wild type ecotype, ii) a T-DNA insertion mutant disrupting the plastoglobule-localized ABC1K6 protein (abc1k6-1) that is delayed in transition to reproductive growth, and iii) a complementation line (Comp3) that restores the timely progression of development with the ABC1K6 wild-type protein sequence tagged C-terminally with GFP in the abc1k6-1 background. Whereas wild-type and abc1k6-1 remain in the vegetative growth stage at 4 weeks of age, the wild-type plants have transitioned to reproductive growth (as evidenced by the emergence of the infloresence bolt) by 5 weeks of age, while abc1k6-1 remians in vegetative growth. Our proteomics experiment seeks to identify leaf proteome differences prior to wild-type transition to reproductive growth that may account for the delayed transition in the abc1k6-1 mutant.