OCA-T1 and OCA-T2 are coactivators of POU2F3 in the tuft cell lineage

ChIP-Seq of C11orf53(OCA-T), COLCA2 and POU2F3(OCT11) in tuft cell lung cancer cell lines. Gene expression changes upon CRISPR knockout of different genes in tuft cell lung cancer cell lines and upon over-expression of C11orf53/POU2F3 alone or in combo in YT330 (a murine Notch high small cell lung cancer cell line) Overall design: ChIP-Seq in NCIH211, NCIH526, CORL311, and NCIH1048 cell lines with endeneous and lentivirally over-expressed construct. Gene expressoin changes in above mentioned cells and YT330 with CRISPR knockout or lentivirally over-expression. Single cell RNA-seq of wild-type and knockout mice.