Differential Effect of Electrical stimuli on a 3D bioprinted model of inflamed skin

To investigate the effects of direct and alternate current on a 3D bioprinted construct containing fibroblasts and keratinocytes in a collagen matrix We then performed gene expression profiling analysis using data obtained from RNA-seq of constructs in physiological and inflamed conditions, using direct or alternate current, at 3 time points Comparative gene expression profiling analysis of RNA-seq data for 3D bioprinted constructs at time 0, 1, and 48 hours after culture, perfused with TNF-α or in physiological conditions, stimulated with direct current (DC) at 1 or 5V, or alternate current (AC) at 10 or 100 Hz, or without stimuli