Two mixed ligand Co(II)-coordination polymers: treatment ability on skin necrosis after fracture and internal fixation by reducing MCSF and TNF-α

Two Co(II)-containing coordination polymers, {Co(TDC)(TPB)_0.5·H₂O}_n (<b>1</b>) and {[Co₂(2,6-NDC)₂(TPB)]·MeCN·3H₂O}_n (<b>2</b>) (TPB = 1,2,4,5-tetra(4-pyridyl)benzene, H₂TDC = 2,5-thiophenedicarboxylic acid, 2,6-H₂NDC = 2,6-naphthalic acid), have been synthesized by a semi-rigid tetrapyridine ligand and auxiliary dicarboxylic ligands. The treatment ability of the compounds on skin necrosis after fracture was assessed and the related mechanism was discussed. First of all, the enzyme-linked immune sorbent assay (ELISA) detection kit was used in this research to detect the content level of the Recombinant Human Macrophage Colony stimulating Factor (MCSF) and Tumor Necrosis Factor-α (TNF-α) after compound treatment. Next, the real time quantitative polymerase chain reaction (RT-PCR) was conducted for the MMP2 and TIMP1 relative expression levels measurement.