RNA-seq for blood Ly6Chi and Ly6Clo monocyte in WT and RBP-Jfl/fl Lyz2-Cre mice

To evaluate the consequences of RBP-J loss of function in blood monocyte subsets, we sequenced RNA of blood Ly6Chi monocyte and Ly6Clo monocyte from WT and RBP-Jfl/fl Lyz2-Cre mice. Blood Ly6Chi and Ly6Clo monocytes were isolated from WT and RBP-Jfl/fl Lyz2-Cre mice, and total RNA was extracted using total RNA purification kit. RNA was converted into RNA-seq quantification libraries, and RNA-seq libraries were sequenced with the pair-end option.为评估RBP-J功能缺失对血液单核细胞亚群的影响，我们对野生型（WT）及RBP-Jfl/fl Lyz2-Cre小鼠的血液Ly6C高表达单核细胞（Ly6Chi monocyte）与Ly6C低表达单核细胞（Ly6Clo monocyte）的RNA进行了测序。我们从野生型及RBP-Jfl/fl Lyz2-Cre小鼠体内分离得到Ly6C高、低表达单核细胞，并使用总RNA纯化试剂盒（total RNA purification kit）提取总RNA；将提取的RNA构建为RNA测序（RNA-seq）定量文库，并采用双端（pair-end）测序模式对文库进行测序。