Expression data from rats post injured tendons 1 week

DNA methylation is involved in many biological activities, such as gene transcription, gene structure stabilization, cell differentiation and foreign body metabolism. Methylation often plays a role in gene silencing in gene regulated transcription. However, the role of DNA methylation of lncRNA in tendon repair is rarely reported. All rats were randomly undertaken control surgery or injured tendons.Tendons were harvested 1 weeks following injured tendons and performed to further analysis. For microarray profiling, the rats tendons total RNA was harvested using TRIzol reagent (Invitrogen, Carlsbad, Canada) and purified using RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol. The amount and quality of RNA were determined using UV-Vis Spectrophotometer (Thermo, NanoDrop 2000, USA) at the absorbance of 260 nm. The mRNA expression profiling was measured using Affymetrix ClariomTM D mouse Array (Affymetrix GeneChip, Santa Clara CA, USA), which contains 65957 gene-level probe sets.