RNA-seq experiment on five mouse brain regions--hypothalamus, hippocampus, neocortex, cerebellum, and the amygdala --comparing expression in virgin females to primiparous females three weeks after pup weaning.

RNA-seq experiment on five mouse brain regions-- --comparing expression in virgin females to primiparous females three weeks after pup weaning. For each brain region there are 8 replicates, though three samples were not used in the final analysis based on inspection of PCA plots (1 sample from the Hippocampus, Cerebellum and Amygdala data sets; see details below). Comparison of gene expression in five brain regions between eight virgin and eight primiparous females three weeks after pup weaning. Pair-wise comparisons were performed using EdgeR transformed data and Limma Voom for statistical analysis.本数据集为针对5个小鼠脑区开展的RNA测序（RNA-seq）实验，旨在比较幼崽断奶三周后未生育雌性小鼠与初产雌性小鼠的基因表达水平。每个脑区最初设置8个生物学重复样本，但经主成分分析（PCA）绘图质控后，有3个样本被排除在最终分析之外（分别来自海马体（Hippocampus）、小脑（Cerebellum）和杏仁核（Amygdala）数据集各1个样本，详见下文）。本研究对比幼崽断奶三周后8只未生育雌性与8只初产雌性在5个脑区中的基因表达差异。统计分析采用EdgeR转换后的数据结合Limma Voom方法开展两两比较与统计学检验。