Human Astrovirus CRISPR screen in Caco2 cells. Human Astrovirus CRISPR screen in Caco2 cells

Though astroviruses are important causes of disease, much of their biology, including cell surface receptors, remains unknown. We used a genome-wide CRISPR knockout library in Caco2 cells to screen for genes important in astrovirus replication. Caco2 CRISPR library cells were infected with human astrovirus 1 and sorted by flow cytometry to identify cells that were not permissive for astrovirus infection. Genomic DNA was extracted and CRISPR guides identified by PCR