G3bp1-miR-1 axis regulates cardiomyocyte hypertrophy

We examine the role of G3bp1, a RNA binding protein and site specific endoribonuclease in gene expression in isolated neonatal cardiomyocytes. RNAseq data from cardiomyocytes were infected with adenoviruses expressing shRNA against G3bp1 (ad-siG3bp1) or Luciferase (ad-siLUC, control) showed significant decrease in transcript abudnnace of cardiac-enriched genes involved in Calcium handling, contraction, action potential and sacromere function. On the other hand increase was observed in genes that regulate Hippo, TNF and TGFb signaling. Knockdown of G3bp1 inhibited endothelin-1 induced cardiomyocyte hypertrophy.本研究探讨了G3bp1——一种RNA结合蛋白与位点特异性核糖核酸内切酶——在分离的新生心肌细胞基因表达中的作用。通过向心肌细胞感染表达靶向G3bp1的短发夹RNA（shRNA）的腺病毒（adenovirus，ad-siG3bp1）或靶向荧光素酶（Luciferase）的对照腺病毒（ad-siLUC），进而获取的RNA测序（RNAseq）数据显示：参与钙离子处理、收缩、动作电位及肌节功能的心脏富集基因的转录本丰度显著降低。与之相反，调控Hippo、肿瘤坏死因子（TNF）及转化生长因子β（TGFβ）信号通路的基因的转录本丰度则显著升高。敲低G3bp1可抑制内皮素-1（endothelin-1）诱导的心肌细胞肥大。