DataSheet_7_Integrative analysis of transcriptome, proteome, and ubiquitome changes during rose petal abscission.xlsx

Plant organ abscission is regulated by multiple physiological and biochemical processes. However, the transcriptional, translational, and post-translational modifications occurring during organ abscission have not been systematically investigated. In this study, we report transcriptome, proteome, and ubiquitome data for the abscission zone (AZ) of rose petals collected during petal shedding. We quantified 40,506 genes, 6,595 proteins, and 2,720 ubiquitinated proteins in rose petal AZ. Our results showed that during petal abscission, 1,496 genes were upregulated and 2,199 were downregulated; 271 proteins were upregulated and 444 were downregulated; and 139 ubiquitination sites in 100 proteins were upregulated and 55 ubiquitination sites in 48 proteins were downregulated. Extracellular levels of cell component proteins were significantly increased, while levels within protoplasts were significantly decreased. During petal abscission, transcript levels of genes involved in defense response, transport, and metabolism changed significantly. Levels of proteins involved in the starch and sucrose metabolism and phenylpropanoid biosynthesis pathways were significantly altered at both the transcript and protein levels. The transcriptional and translational upregulation of peroxidase (POD), in the phenylpropanoid biosynthesis, pathway may be associated with deposition of lignin, which forms a protective layer during petal abscission. Overall, our data provide a comprehensive assessment of the translational and post-translational changes that occur during rose petal abscission.

植物器官脱落受到多种生理和生化过程的调控。然而，在器官脱落过程中发生的转录、翻译及翻译后修饰尚未得到系统性的研究。在本研究中，我们报道了在花瓣脱落过程中收集到的玫瑰花瓣脱落区（AZ）的转录组、蛋白质组和泛素化蛋白质组数据。我们量化了玫瑰花瓣AZ中的40,506个基因、6,595个蛋白质和2,720个泛素化蛋白质。我们的结果表明，在花瓣脱落过程中，1,496个基因表达上调，而2,199个基因表达下调；271个蛋白质表达上调，而444个蛋白质表达下调；在100个蛋白质中的139个泛素化位点表达上调，而在48个蛋白质中的55个泛素化位点表达下调。细胞组分蛋白质的细胞外水平显著增加，而细胞质内水平显著降低。在花瓣脱落过程中，参与防御反应、运输和代谢的基因的转录水平发生了显著变化。参与淀粉和蔗糖代谢以及苯丙烷类生物合成途径的蛋白质水平在转录和蛋白质水平上均发生了显著改变。在苯丙烷类生物合成途径中，过氧化物酶（POD）的转录和翻译上调可能与木质素的沉积有关，木质素在花瓣脱落过程中形成保护层。总体而言，我们的数据为玫瑰花瓣脱落过程中发生的翻译和翻译后变化提供了全面的评估。