Brain-muscle tissue communication prevents muscle aging by maintaining daily physiology

The muscle peripheral clock selects, suppresses, and integrates signals from the central clock that serve muscle homeostasis, thus revealing its critical gatekeeper function. Four biological replicates (2 males and 2 females, if not otherwise specified) at each zeitgeber time (ZT) point were used to collect RNA samples from the snap-frozen tibialis anterior muscle. A total of 360 mouse RNA samples were sequenced to generate the circadian RNA transcriptome. Eight samples were excluded from analysis due to quality issues.肌肉外周生物钟（muscle peripheral clock）可筛选、抑制并整合来自中枢生物钟（central clock）的、服务于肌肉稳态（muscle homeostasis）的信号，由此展现出其关键的把关者功能。本研究在每个光照周期时间（zeitgeber time, ZT）点设置4个生物学重复（biological replicates，若无特殊说明，均为2只雄性与2只雌性小鼠），用于从快速冷冻的胫骨前肌中采集RNA样本；共对360份小鼠RNA样本进行测序以获取昼夜节律RNA转录组（circadian RNA transcriptome）数据，其中8份样本因质量问题被排除于后续分析之外。