Digital RNA-Seq for transcriptome analysis in splenic human CD4-positive T cells of mock-infected humanized mice and GFP-positive and GFP-negative CD4-positive cells of HIV1-GFP-infected humanized mice

Transcriptome analysis of digital RNA-sequencing (dRNA-Seq) data reveals the differentially expressed genes between GFP-positive and GFP-negative cells. GFP-positive cells and GFP-negative cells were sorted from the splenic human CD4-positive T cells of HIV1-GFP-infected humanized mice (n=6) at 2 weeks post-infection. The splenic human CD4-positive T cells were also sorted from mock-infected mice (n=7). By using these samples, dRNA-Seq was performed to performe transcriptome analysis (3 technical replicates for 3 infected mice and 3 mock-infected mice; 1 technical replicate for 3 infected mice and 4 mock-infected mice).