RNA-seq analysis for mRNA expression change in oxygen/glucose deprivation-treated cultured oligodendrocyte precursor cells

Oligodendrocyte precursor cells (OPCs) regulate neuronal, glial and vascular systems in diverse ways and display phenotypic heterogeneity beyond their well-known role as a reservoir for mature oligodendrocytes. Here we demonstrated that perivascular OPCs in cerebral cortex were increased coupling with post-stroke angiogenesis in a mouse model of stroke induced by middle cerebral artery occlusion (MCAO). RNA-seq analysis revealed that primary cultured OPCs notably increased the gene expressions of numerous pro-angiogenic factors, while decreased those related to OPC maturation after oxygen glucose deprivation. The data showed that cerebral ischemia shifts the phenotype of OPCs to perivascular subtypes which can promote angiogenesis.少突胶质前体细胞（Oligodendrocyte precursor cells, OPCs）可通过多种方式调控神经元、胶质细胞与血管系统，且除了作为成熟少突胶质细胞储备库这一广为人知的功能外，还表现出表型异质性。本研究在大脑中动脉闭塞（middle cerebral artery occlusion, MCAO）诱导的小鼠脑卒中模型中证实，大脑皮层的血管周围少突胶质前体细胞数量增加，且该变化与脑卒中后血管生成过程相耦合。RNA测序（RNA-seq）分析显示，经氧糖剥夺处理后，原代培养的少突胶质前体细胞显著上调多种促血管生成因子的基因表达，同时下调与OPC成熟相关的基因表达。本研究数据表明，脑缺血可将少突胶质前体细胞的表型转变为血管周围亚型，而这类亚型能够促进血管生成。