scRNA-seq of tumor-infiltrating CD45+ cells from B16-F10 tumor-bearing mice treated with vehicle and DOPAC.

To investigate how DOPAC suppresses tumors in vivo, we performed single-cell RNA sequencing (scRNA-seq) on CD45+ cells isolated from the tumor tissues of B16-F10-bearing mice. To perform single-cell RNA sequencing analysis, we treated mice under each treatment condition (Vehicle-treated and DOPAC-treated, n=4 biological replicates/group). Flow sorting was used to obtain tumor-infiltrating CD45+ cells and cell suspensions were dissociated and loaded into the 10x Chromium instrument.