Proteins on exocytic vesicles mediate calcium-triggered fusion.
收藏PubMed Central1992-05-15 更新2026-05-16 收录
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https://pmc.ncbi.nlm.nih.gov/articles/PMC49161/
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资源简介：
In many exocytic systems, micromolar concentrations of intracellular Ca2+ trigger fusion. We find that aggregates of secretory granules isolated from sea urchin eggs fuse together when perfused with greater than or equal to 10 microM free Ca2+. Mixing of membrane components was demonstrated by transfer of fluorescent lipophilic dye, and melding of granule contents was seen with differential interference microscopy. A technique based upon light scattering was developed to conveniently detect fusion. Two protein modifiers, trypsin and N-ethylmaleimide, inhibit granule-granule fusion at concentrations similar to those that inhibit granule-plasma membrane fusion. We suggest that molecular machinery sufficient for Ca(2+)-triggered fusion resides on secretory granules as purified and that at least some of these essential components are proteinaceous. IMAGES:
提供机构：
National Academy of Sciences
创建时间：
1992-05-15
