Tracing colonic embryonic transcriptional programs and their reactivation in inflammatory disease at single cell level

Purpose: determine transcriptional programs active during hindgut development and their reactivation in inflammatory disease; Summary: Our analyses reveal that most adult cell lineages are established by the late embryonic stage. In addition we find reactivation of embryonic features in inflammatory disease. All embryonic (E14.5, E15.5, E18.5) and adult (DSS treatment) cells were sorted using FACS (using EpCAM and CD45 antibodies). Single cell RNA sequencing was performed (for details see Fazilaty et al, in prep)研究目的：解析后肠发育过程中活跃的转录程序，以及该程序在炎症性疾病中的再激活现象。 研究概述：本研究的分析结果显示，绝大多数成体细胞谱系在胚胎发育晚期即已建立；此外还观察到，炎症性疾病中存在胚胎特征的再激活过程。所有胚胎期（E14.5、E15.5、E18.5）与成年期（经葡聚糖硫酸钠（Dextran Sulfate Sodium, DSS）处理）的细胞，均通过荧光激活细胞分选术（Fluorescence-Activated Cell Sorting, FACS）完成分选，分选所用抗体为上皮细胞黏附分子（EpCAM）与CD45抗体。后续开展了单细胞RNA测序（Single Cell RNA Sequencing），详细实验方法参见Fazilaty等人的待发表研究。