These files provide the supplementary data supporting Dempster et al., "Agreement between two large pan-cancer CRISPR-Cas9 gene dependency datasets." They represent the results of two genome-scale CRI

Figshare2019-04-10 更新20

Straightforward Delivery of Linearized Double-Stranded DNA Encoding sgRNA and Donor DNA for the Generation of Single Nucleotide Variants Based on the CRISPR/Cas9 System

基因递送系统

Cas9蛋白

CRISPR/Cas9 for genome editing requires delivery of a guide RNA sequence and donor DNA for targeted homologous recombination. Typically, single-stranded oligodeoxynucleotide, serving as the donor temp

NIAID Data Ecosystem50

Linear plasmid 28–1 (lp28-1) retention in B . burgdorferi following Cas9 targeting, as detected by plating.

质粒稳定性

Cas9蛋白

Linear plasmid 28–1 (lp28-1) retention in B. burgdorferi following Cas9 targeting, as detected by plating.

NIAID Data Ecosystem20