Male rats emit aversive 44-kHz ultrasonic vocalizations during prolonged Pavlovian fear conditioning. Olszyński, Polowy et al.

Wistar and SHR rats underwent fear conditioning (FC) with 0, 1, 6 or 10 shocks (1 s, 1.0 mA each). We have observed the rats which received 6-10 shocks, especially, to exhibit ultrasonic vocalizations (USV) which were long, flat – i.e., of constant frequency – usually within 35-50 kHz range. We named them 44-kHz USV. Here are raw data showing rats’ behavior during fear conditioning (FC) and USV playback sessions. The dataset is related to the publication in eLife by K.H. Olszynski, R. Polowy and others (DOI: 10.7554/eLife.88810), and includes four types of files:
1. Files in .xlsx format (5 folders, A–E),
2. Files in .wav format (10 recordings).
3. Files in .csv format (DBSCAN folder)
4. Files in .mat format (k-means folder)
The files are accompanied by their description.
1.
A. FOLDER "FC_freezing, USV (10-s-intervals)" contains data from FC: freezing and number of emitted USV, presented in 10-s intervals.
B. FOLDER "FC_USV" contains data from fear conditioning: emitted USV (properties of individual USV).
C. FOLDER "Playback_distance, time, USV (10-s-intervals)" contains data from playback sessions (distance traveled, time spent in the half of the cage with the speaker, heart rate, number of emitted USV and 50-kHz USV), presented in 10-s intervals, with additional analysis of USV properties emitted in response to playback.
D. FOLDER "Playback_USV" contains data from playback sessions: properties of individual USV emitted in response to ultrasonic playback.
E. FOLDER “Data from selected rats” contains 3 files:
E.1. Step up and step down frequencies
Frequencies for assessing 22-kHz-elements vs. 44-kHz-elements frequency ratio:
E.2. Freezing during USV emission (data for Table 2)
Percent of freezing during 22-kHz and 44-kHz USV
E.3. Mean power (data for Figure 1–figure supplement 2)
Mean power, frequency, and category of USV,

2. Files in .wav format. Two selected recordings, registered during fear conditioning sessions:
2.1. FC_training_Wistar_delay_6-T_#63-6. This rat is also featured in Figure 1—figure supplement 1C (top row, first from the left).
2.2. FC_training_Wistar_trace_10-T_#P44.
2.3. Recordings used for playback experiments:
1. 44-kHz long calls
2. 22-kHz long calls
3. 22-kHz short modulated calls
4. 22-kHz short flat calls
5. 50-kHz modulated calls
6. 50-kHz flat calls
7. 50-kHz trill calls
8. 50-kHz mixed calls

3. Files in .csv format (DBSCAN folder); data supporting clustering files for DBSCAN algorithm (.csv) used in Figure 3A. File names contain: experiment number (E1–3), FC (for “fear conditioning”), rat strain (W, Wistar; SHR), rat group (S, single; P, paired; T0, 0-Trial; T1, 1-Trial; T6, 6-Trial; T10, 10-Trial), rat individual number.

4. Files in .mat format (k-means folder) contain extracted call contours used for k-means algorithm (.mat) and visualized in Figure 3BC, Figure 3—figure supplement 1.

Wistar 及 SHR 大鼠接受了0、1、6或10次电击（每次1秒，1.0毫安）的恐惧条件化（FC）训练。我们观察到，接受6-10次电击的大鼠，尤其是表现出长而平直的超声波发声（USV），即频率恒定的发声，通常在35-50千赫兹的范围内。我们将其命名为44千赫兹USV。以下为恐惧条件化（FC）过程中大鼠行为及USV回放会话的原始数据。该数据集与K.H. Olszynski、R. Polowy等人在eLife杂志上发表的论文（DOI: 10.7554/eLife.88810）相关，包含四种类型的文件：

1. .xlsx格式的文件（A-E五个文件夹），
2. .wav格式的文件（10个录音），
3. .csv格式的文件（DBSCAN文件夹），
4. .mat格式的文件（k-means文件夹）。文件附有相应的描述。

1. 文件夹“A”
A. “FC_freezing, USV (10-s-intervals)”文件夹包含恐惧条件化过程中的冻结状态和发出的USV数量数据，以10秒为间隔呈现。
B. “FC_USV”文件夹包含恐惧条件化过程中发出的USV数据（单个USV的性质）。
C. “Playback_distance, time, USV (10-s-intervals)”文件夹包含回放会话数据（移动距离、在包含扬声器的一半笼子中度过的时间、心率、发出的USV数量和50千赫兹USV），以10秒为间隔呈现，并附加了针对回放过程中发出的USV性质的分析。
D. “Playback_USV”文件夹包含回放会话中发出的单个USV性质数据。
E. “Data from selected rats”文件夹包含3个文件：
E.1. 上升和下降频率
评估22千赫兹元素与44千赫兹元素频率比的频率
E.2. 发声期间的冻结状态（表2数据）
22千赫兹和44千赫兹USV期间冻结的百分比
E.3. 平均功率（图1-图补充2数据）
USV的平均功率、频率和类别

2. .wav格式的文件。两份在恐惧条件化过程中记录的选定录音：
2.1. FC_training_Wistar_delay_6-T_#63-6。该大鼠也出现在图1-图补充1C（顶部行，从左数第一个）。
2.2. FC_training_Wistar_trace_10-T_#P44。
2.3. 用于回放实验的录音：
1. 44千赫兹长呼叫声
2. 22千赫兹长呼叫声
3. 22千赫兹短调谐呼叫
4. 22千赫兹短平呼叫
5. 50千赫兹调谐呼叫
6. 50千赫兹平呼叫
7. 50千赫兹颤音呼叫
8. 50千赫兹混合呼叫

3. .csv格式的文件（DBSCAN文件夹）包含支持DBSCAN算法聚类的文件（.csv），用于图3A中的聚类文件。文件名包含：实验编号（E1-3）、FC（恐惧条件化）、大鼠品系（W、Wistar；SHR）、大鼠组（S、单个；P、成对；T0、0次试验；T1、1次试验；T6、6次试验；T10、10次试验）、大鼠个体编号。

4. .mat格式的文件（k-means文件夹）包含用于k-means算法的提取呼叫轮廓（.mat）并在图3BC、图3-图补充1中可视化的文件。