lncRNA GAS5 and its effect

The aim of the study was to explore the serum expression of long non-coding RNA (lncRNA) growth arrest-specific transcript 5 (GAS5) in Mycoplasma pneumoniae pneumonia (MPP) and its effect on LAMPS-induced apoptosis and inflammation. Totally, 56 children with MPP (MPP group) and 56 healthy children (NC group) were enrolled. The lncRNA GAS5 expression was measured by qRT-PCR. Serum levels of tumor necrosis factor α (TNF-α) and interleukin-6 (IL-6) were detected using ELISA, and the high mobility family protein B1 (HMGBl) was detected by qRT-PCR. The methylated binding protein 2 (MECP2) was inhibited by gene silencing, and the expression of MECP2, TNF-α, IL-6, HMGBl, p-p65, and p-IκBα was measured. The lncRNA GAS5 and TNF-α, IL-6, and HMGBl in the peripheral blood of the MPP group was positively correlated (P<0.05). The expression of TNF-α, IL-6, HMGBl, and lncRNA GAS5 showed a positive correlation with that of LAMPS. The GAS5-siRNA group showed increased cell survival rate compared with the scrambled-RNAi group (P< 0.05), while showed decreased apoptosis and cell death rates (P< 0.05). Additionally, the expression of IL-6, TNF-α, HMGBl, p-p65, and p-IκBα was significantly reduced (P< 0.05). lncRNA GAS5 is highly expressed in the serum of children with MPP and inhibits LAMPS-induced apoptosis and alveolar macrophage inflammation.