Monosynaptic inputs to dopamine neurons in the ventral tegmental area and substantia nigra compacta and serotonin neurons in the dorsal raphe and median raphe

Monosynaptic inputs to serotonin neurons in the dorsal raphe (DR) and median raphe (MnR) were labeled in mice using the modified rabies virus SADdG-GFP (envA). The anatomical location and corresponding brain regions of labeled input neurons were systematically recorded and stored using MATLAB. This dataset includes the extracted input mapping data, along with the custom MATLAB codes used for data extraction, processing, and analysis of the retrograde tracing experiments.
, Virus for Cre-dependent expression of receptor (AAV5-FLEX-TVA-mCherry) and rabies G protein (AAV8-FLEX-RG) were injected into the dorsal raphe (DR) or median raphe (MnR) of serotonin transporter (Sert)-Cre mice. 2 weeks later, pseudotyped modified rabies virus (SADdG-GFP(envA)) was injected at the same location. The brain was fixed with phosphate-buffered saline (PBS) and paraformaldehyde (PFA) serial perfusion, and cut into 100 μm coronal sections. Every 3 sections of the obtained brain sections were counter-stained with NeuroTrace Fluorescent Nissl Stains (Molecular Probes) and imaged with Zeiss AxioImager Z2 using a 10x lens. GFP-labeled neurons were manually registered onto brain areas based on Paxinos and Franklin’s atlas.
, # Monosynaptic inputs to dopamine neurons in the ventral tegmental area and substantia nigra compacta, and serotonin neurons in the dorsal raphe and median raphe

Dataset DOI: [10.5061/dryad.2bvq83c4n](https://doi.org/10.5061/dryad.2bvq83c4n)

## Description of the data and file structure

Each zip file (e.g., DRf1.zip) contains all original TIF images and extracted data MAT files for each animal. “DRf*” is tracing from serotonin neurons in the dorsal raphe (DR), and “MRf*” is tracing from serotonin neurons in the median raphe (MnR). The original starter neurons and their monosynaptic inputs are labeled with green (GFP), and the Nissl counterstaining is labeled with red. The images are taken with an x10 objective lens, and one pixel in the MAT files corresponds to 2.6871 x 10^-3^ mm.

### Files and variables

Data in zip folders

#### Each .mat file contains one structure “analy”.

analy.Rat: animal name

analy.Section: brain section number

analy.Bregma: the estimated anterior-posterio...,