Derivation of hypoblast stem cells from rat embryonic stem cells. [Affymetrix]

Hypoblast stem cells were derived from rat embryonic stem cells (rESCs) by culturing with LIF, EGF, PDGF, and 2% fetal bovine serum. mRNA expression profiles of the cells during derivation at different time points were determined with Affymetrix microarrays. Global transcriptome of rESC-derived hypoblast stem cells (cHypoSCs) was similar to that of rat embryo-derived hypoblast stem cells and rat multipotent adult progenitor cells. In addition, the transcriptome of these cells were compared with the previously published datasets including transcriptome of mouse embryonic stem cells, extraembryonic endoderm cells, single cells of mouse embryos (E3.25, E3.5, and E4.5). The comparison revealed that cHypoSCs have the transcriptome similar to that of extraembryonic endoderm cells and E3.5 primitive endoderm cells. Cells transitioning from rESCs to cHypoSCs (day 0, day 7, day 17, and day 32) were prepared. rESCs grown on feeder-free and feeder-dependent culture were used for derivation of cHypoSCs, and the cells from each condition were used. RNA was extracted from these cells. Then, the samples were hybridized to GeneChip Rat Gene 1.0 ST array (Affymetrix).