Differential gene expression in TREM1 -/- vs +/+ myeloid cells 48h after MCAo-RP

Stroke is a multiphasic progress characterized by neuron damage due to hypoxia followed by secondary damage from the subsequent inflammatory immune response. Infiltrating myeloid cells induce cerebral damage through pro-inflammatory cytokines, chemokines, proteases and generation of reactive oxygen species (ROS). Triggering receptor expressed on myeloid cells 1 (TREM1) is exclusively expressed on myeloid cells and acts as an amplifier of pro-inflammatory innate immune responses. Using microarray analysis, we aim to identify the expression differences and functional change of these myeloid cells after TREM1 knockout in mice post stroke. TREM1-/- vs +/+ C57B6/J male mice underwent transient cerebral ischemia (MCAo-RP). At 48 hours after MCAo-RP, myeloid cells in ischemic hemisphere were isolated, stained with biomarkers (CD45, CD11b, and Ly6g) and sorted immediately by Fluorescence-activated cell sorting (FACS). Affimetrix Clariom microarray was used to examine differential gene expression on microglia, macrophages, and neutrophils from ischemic hemisphere.