Influence of CPM-dependent sorting on the multi-omics profile of hepatocyte-like cells matured in microscale biochips.

We investigated the transcriptome of human induced pluripotent stem cells (hiPSCs) differentiated into hepatocyte-like cells (HLCs) in a biochip culture environment with or without carboxypeptidase M positive (CPM+) cells selection using nanoCAGE, a method for promoters, transcription factors, and transcriptome analysis. Comparison of the multi-omics profiles of human induced pluripotent stem cells (hiPSCs) differentiated into hepatocyte-like cells (HLCs) in a biochip culture environment either with or without applying a carboxypeptidase M positive (CPM+) cells sorting step before cell inoculation.