Time course of the miR-203 effect on the transcriptomic profile of IPSCs

The balance between pluripotency and differentiation is critical during development and regeneration. miR-203 is a microRNA previously involved in differentiation of different tissues as well as in tumor suppression in multiple malignancies. We have shown that miR-203 is able to promote differentiation of embryonic stem (ES) and induced pluripotent stem (iPS) cells without decreasing pluripotency. We have observed that transient expression of miR-203 significantly improves the efficiency of ES/iPS cells in the generation of quimeras and tetraploid complementation assays, in addition to inducing complex embryo-like structures when these pluripotent cells are injected in mice. In the present RNA seq, we intend to analyze the trascriptomic profile of WT IPSCs transiently treated with DOX to induce miR-203. We analyzed the transcriptomic profile at different time points before and after the induction. The general idea was to analyze the transcriptomic profile of miR-203 transiently over-expressing IPSCs, and compare different time points, from t=0 (before DOX treatment) to embryoid bodies formation. miR-203 tKI IPSCs were transiently induced by doxycycline treatment for 5 days, and then the following time points were analyzed: t=10 (5 days after DOX treatment); t=25 (20 days after DOX treatment) and t=32 (7 days after embryoid body differentiation).