A C. elegans LSD1 Demethylase Contributes to Germline Immortality by Reprogramming Epigenetic Memory

Microarray-based expression profiling of mixed stage populations taken from generation 1,13 and 26 spr-5 mutant animals as well as wild-type animals reveals a large class of spermatogenesis-expressed genes whose expression coordinately increased from generations 1 to 13 and then decreased from generations 13 to 26 in spr-5(by101) mutants. These results suggest that a failure to reset spermatogenesis acquired H3K4me2 may result in the progressive sterility that is observed in spr-5 mutants.基于微阵列（Microarray）的表达谱分析，对取自第1、13和26代spr-5突变体动物以及野生型动物的混合发育阶段种群进行检测，结果发现在spr-5(by101)突变体中存在一大类精子发生表达基因，其表达水平在第1代至第13代间协同上调，并在第13代至第26代间协同下调。上述结果表明，无法重置精子发生过程中获得的组蛋白H3赖氨酸4二甲基化（H3K4me2），可能会导致spr-5突变体中观察到的进行性不育。