Multi-axial self-organisation properties of mouse embryonic stem cells into gastruloids [E6.5 to E9.5]. Multi-axial self-organisation properties of mouse embryonic stem cells into gastruloids [E6.5 to E9.5]

Gastruloids are small aggregates of murine Embryonic Stem (ES) cells stimulated to undergo gastrulation-like symmetry breaking events in vitro (van den Brink, S. C. et al. 2014, Simunovic, M. & Brivanlou, A. H 2017). We analyzed the transcriptomic profiles of gastruloids at different time-points after aggregation (AA) and compared them with those of mouse embryo at different stages, raging from e6 to e9.5. We show that gastruloids are capable of organizing a post-occipital pattern of neural, mesodermal and endodermal derivatives that mimics that of the embryo in terms of gene expression. Overall design: RNA-seq analysis of wild type ouse embryos at different developmental stages ranging from E6.5 to E9.5