Myelodysplastic Syndrome (MDS) in Humanized Mice

This study was to develop a faithful pre-clinical MDS patient-derived xenotransplantation model in cytokine-humanized immunodeficient "MISTRG" mice. We performed targeted genome sequencing on bone marrow from patients with MDS or AML and from bone marrow of mice engrafted with the respective patient bone marrow. Cytokine-humanized immunodeficient mice faithfully replicate human MDS.]]> Patients with MDS were prospectively identified for enrollment into tissue analysis and banking cohorts (Yale University). Eligible patients included those with known as well as those with newly diagnosed MDS. After obtaining informed consent for sample collection and genomic analysis of their blood and/or bone marrow, blood blood/bone marrow were collected as part of their routine clinical care. All patients provided written informed consent to allow the collection of bone marrow and blood and analysis of clinical and genetic data for research purposes (Yale University).]]>